ADAR Antibody (OAGA00107)

Catalog No: OAGA00107

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
OAGA00107 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A:NIH-3T3 5% SDS PAGE
OAGA00107 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Colon ca, using ADAR1(OAGA00107) antibody at 1:250 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ADAR1(OAGA00107) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS, 1% BSA, 20% Glycerol (pH7) with 0.01% Thimerosal.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB, IP
• Additional Information: Positive Controls: 293T, NIH-3T3
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 472 and 757 of ADAR1 (Uniprot ID#P55265)
• Purification: Purified by antigen-affinity chromatography
• Predicted Homology Based on Immunogen Sequence: Rat
• Application Info: WB 1:500~1:3000
  IP 1:100~500
  IHC-P 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]
  ICC/IF 1:100~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: ADAR
• Gene Full Name: Double-stranded RNA-specific adenosine deaminase
• Alias Symbols: DSH, AGS6, G1P1, IFI4, P136, ADAR1, DRADA, DSRAD, IFI-4, K88DSRBP
• NCBI Gene Id: 103
• Protein Name: Double-stranded RNA-specific adenosine deaminase
• Description of Target: This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Uniprot ID: P55265
• Protein Accession #: NP_001020278.1
• Molecular Weight: 136 kDa