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Catalog No: OKEH03412
Size:96T
Price: $525.00
SKU
OKEH03412
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ADAMTS4 ELISA Kit (Rat) (OKEH03412) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adamts4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adamts4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adamts4 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Adamts4 Microplate96 Wells (12 x 8 Well strips)
Adamts4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Adamts4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity40.4 pg/mL
SpecificityNatural and recombinant Rat A disintegrin and metalloproteinase with thrombospondin motifs 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAdamts4
Gene Full NameADAM metallopeptidase with thrombospondin type 1 motif, 4
Alias Symbolsa disintegrin and metallopeptidase with thrombospondin motifs 4;A disintegrin and metalloproteinase with thrombospondin motifs 4;a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4;ADAM-TS 4;ADAMTS-4;ADAM-TS4;Aggrecanase;aggrecanase-1.
NCBI Gene Id66015
Protein NameQ9ESP7
Description of TargetA disintegrin and metalloproteinase with thrombospondin motifs 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q9ESP7
Protein Accession #NP_076449.1
Nucleotide Accession #NM_023959.1
Protein Size (# AA)630
Molecular Weight68 kDa
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