ADAMDEC1 ELISA Kit (Human) (OKCA01092)

Catalog No: OKCA01092

Size:96 Wells

Human ADAMDEC1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	92	87-96
  1:2	96	90-101
  1:4	94	89-99
  1:8	97	92-103

• ELISA Kit Principle: Aviva Systems Biology ADAMDEC1 ELISA Kit (Human) (OKCA01092) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMDEC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMDEC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ADAMDEC1 captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	104	99-108
  EDTA Plasma (n=4)	94	89-98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01092 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01092 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • ADAMDEC1 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAMDEC1 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated ADAMDEC1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: ADAMDEC1
• Gene Full Name: ADAM like decysin 1
• Alias Symbols: M12.219
• NCBI Gene Id: 27299
• Protein Name: ADAM DEC1
• Description of Target: May play an important role in the control of the immune response and during pregnancy.
• Uniprot ID: O15204
• Protein Accession #: NP_001138743.1
• Nucleotide Accession #: NM_001145271.1