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Catalog No: OKCD02341
Size:96WELLS
Price: $625.00
SKU
OKCD02341
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Datasheets/ManualsPrintable datasheet for ADAM8 ELISA Kit (Mouse) (OKCD02341)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-105%93-105%89-104%98-105%
EDTA Plasma (n=5)79-105%89-103%93-102%85-102%
Heparin Plasma (n=5)78-98%78-91%78-101%86-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 8 (ADAM8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 8 (ADAM8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 8 (ADAM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of A Disintegrin And Metalloprotease 8 (ADAM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)94-10299
EDTA Plasma (n=5)92-10196
Heparin Plasma(n=5)84-9895
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 8 (ADAM8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 8 (ADAM8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ADAM8 Microplate96 Wells (12 x 8 Well strips)
ADAM8 Lyophilized Standard2
100X Biotinylated ADAM8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity3.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 8 (ADAM8). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 8 (ADAM8) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAdam8
Gene Full Namea disintegrin and metallopeptidase domain 8
Alias Symbolsa disintegrin and metalloprotease domain 8;ADAM 8;CD156;CD156a;cell surface antigen MS2;disintegrin and metalloproteinase domain-containing protein 8;E430039A18Rik;macrophage cysteine-rich glycoprotein;MS;MS2.
NCBI Gene Id11501
Protein NameDisintegrin and metalloproteinase domain-containing protein 8
Description of TargetPossible involvement in extravasation of leukocytes.
Uniprot IDQ05910
Protein Accession #NP_031429.1
Nucleotide Accession #NM_007403.3
Protein Size (# AA)826
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