Catalog No: OKEH02363
Size:96T
Price: $625.00
SKU
OKEH02363
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02363
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ADAM28 ELISA Kit (Human) (OKEH02363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM28 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM28 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM28 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
ADAM28 Microplate96 Wells (12 x 8 Well strips)
ADAM28 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ADAM28 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Disintegrin and metalloproteinase domain-containing protein 28
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolADAM28
Gene Full NameADAM metallopeptidase domain 28
Alias SymbolsADAM 28;disintegrin and metalloproteinase domain-containing protein 28;eMDC II;eMDCII;epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;epididymis secretory sperm binding protein;MDCL;MDC-L;Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L;metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L.
NCBI Gene Id10863
Protein NameQ9UKQ2
Description of TargetDisintegrin and metalloproteinase domain-containing protein 28
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UKQ2
Protein Accession #NP_001291280.1
Nucleotide Accession #NM_001304351.1
Protein Size (# AA)775
Molecular Weight87 kDa
Write Your Own Review
You're reviewing:ADAM28 ELISA Kit (Human) (OKEH02363)
Your Rating
We found other products you might like!