ADAM17 ELISA Kit (Human) : 96 Wells (OKEH00945)

Catalog No: OKEH00945

Size:96T

Human ADAM17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ADAM17 ELISA Kit (Human) (OKEH00945) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM17 captured in well.
  
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 10.3% (n = 20)
• ELISA Kit Component:
  • ADAM17 Microplate: 96 Wells (12 x 8 Well strips)
  • ADAM17 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated ADAM17 Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.11 ng/mL
• Specificity: Natural and recombinant Human Disintegrin and metalloproteinase domain-containing protein 17
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ADAM17
• Gene Full Name: ADAM metallopeptidase domain 17
• Alias Symbols: a disintegrin and metalloproteinase 17;ADAM metallopeptidase domain 18;ADAM18;cartilage snake venom-like protease;CD156B;CSVP;disintegrin and metalloproteinase domain-containing protein 17;NISBD;NISBD1;snake venom-like protease;TACE;TNF-alpha convertase;TNF-alpha convertase enzyme;TNF-alpha converting enzyme;TNF-alpha-converting enzyme;tumor necrosis factor, alpha, converting enzyme.
• NCBI Gene Id: 6868
• Protein Name: P78536
• Description of Target: Disintegrin and metalloproteinase domain-containing protein 17
• Uniprot ID: https://www.uniprot.org/uniprot/P78536
• Protein Accession #: NP_003174.3
• Nucleotide Accession #: NM_003183.5
• Protein Size (# AA): 824
• Molecular Weight: 93 kDa