ADAM10 ELISA Kit (Mouse) (OKEH06827)

Catalog No: OKEH06827

Size:96T

Mouse ADAM10 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Adam10 ELISA Kit (Mouse) (OKEH06827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adam10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adam10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adam10 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-Assay Reproducibility CV% < 7.1% (n=20)< br>Inter-Assay Reproducibility CV% < 9.2% (n=24)
• ELISA Kit Component:
  • SECISBP2L Microplate: 96 Wells (12 x 8 Well strips)
  • SECISBP2L Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SECISBP2L Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.081 ng/mL
• Specificity: Natural and recombinant Mouse Disintegrin and metalloproteinase domain-containing protein 10
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Adam10
• Gene Full Name: a disintegrin and metallopeptidase domain 10
• Alias Symbols: 1700031C13Rik;a disintegrin and metalloprotease domain (ADAM) 10;disintegrin and metalloproteinase domain-containing protein 10;kuz;kuzb;kuzbanian;kuzbanian protein homolog;MADM;mammalian disintegrin-metalloprotease.
• NCBI Gene Id: 11487
• Protein Name: O35598
• Description of Target: Disintegrin and metalloproteinase domain-containing protein 10
• Uniprot ID: https://www.uniprot.org/uniprot/O35598
• Protein Accession #: NP_031425
• Nucleotide Accession #: NM_007399