ADAL ELISA Kit (Human) (OKEH01830)

Catalog No: OKEH01830

Size:96T

Human ADAL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ADAL ELISA Kit (Human) (OKEH01830) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ADAL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ADAL and incubated. The ADAL found in the sample or standards competes with the biotinylated ADAL for limited binding sites on the immobilized anti-human ADAL antibody. Excess unbound biotinylated ADAL and sample or standard ADAL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ADAL captured in the well and inversely proportional to the amount of ADAL which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-ADAL Microplate: 96 Wells (12 x 8 Well strips)
  • ADAL Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ADAL-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Human Adenosine deaminase-like protein
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ADAL
• Gene Full Name: adenosine deaminase like
• Alias Symbols: adenosine deaminase-like protein;HsMAPDA;N6-mAMP deaminase;N6-methyl-AMP aminohydrolase.
• NCBI Gene Id: 161823
• Protein Name: Q6DHV7
• Description of Target: Adenosine deaminase-like protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q6DHV7
• Protein Accession #: NP_001012987.1
• Nucleotide Accession #: NM_001012969.3
• Protein Size (# AA): 355
• Molecular Weight: 40 kDa