Catalog No: OKCD00262 (Formerly GWB-KBBHH2)
Size:96WELLS
Price: $650.00
SKU
OKCD00262
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Datasheets/ManualsPrintable datasheet for ACVR1 ELISA Kit (Human) (OKCD00262)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type I (ACVR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type I (ACVR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type I (ACVR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Activin A Receptor Type I (ACVR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A Receptor Type I (ACVR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A Receptor Type I (ACVR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ACVR1 Microplate96 Wells (12 x 8 Well Strips)
ACVR1 Lyophilized Standard2 x 10 ng
100X Biotinylated ACVR1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type I (ACVR1). No significant cross-reactivity or interference between Activin A Receptor Type I (ACVR1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolACVR1
Gene Full Nameactivin A receptor type 1
Alias Symbolsactivin A receptor, type I;activin A receptor, type II-like kinase 2;activin receptor type I;activin receptor type-1;activin receptor-like kinase 2;ACTRI;ACVR1A;ACVRLK2;ALK2;FOP;hydroxyalkyl-protein kinase;serine/threonine-protein kinase receptor R1;SKR1;TGF-B superfamily receptor type I;TSRI.
NCBI Gene Id90
Protein NameActivin receptor type-1
Description of TargetOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis (By similarity).
Uniprot IDQ04771
Protein Accession #NP_001096
Nucleotide Accession #NM_001105.4
Protein Size (# AA)509
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