ACTB Antibody (OAAD00224)

Catalog No: OAAD00224

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized from 1.2% sodium acetate, with 2 mg/ml BSA and 0.01 mg sodium azide
• Clonality: Monoclonal
• Clone: AC-15
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP
• Reconstitution and Storage: Reconstitute with 1.2% sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 ug/mL. Store at -20°C for one year. After reconstitution, at 4°C for three months. It can also be aliquotted and stored frozen at -20°C for a longer time.
• Immunogen: Slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Application Info: WB: 0.25 - 0.5 ug/mL with the appropriate system to detect beta-actin in cells and tissues
  IHC-P: 0.5 - 1 ug/mL to detect beta-actin in formalin fixed and paraffin embedded tissues.
• Publications:

  Selective cytoprotective effect of histamine on doxorubicin-induced hepatic and cardiac toxicity in animal models. Cell Death Discov. 1, 15059 (2015). 27551485

• Reference: 1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985.
  2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006.
  3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.

Target Info

• Gene Symbol: ACTB
• Gene Full Name: Actin, cytoplasmic 1
• Alias Symbols: BRWS1, PS1TP5BP1
• NCBI Gene Id: 60
• Protein Name: Actin, cytoplasmic 1
• Description of Target: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
• Uniprot ID: P60709
• Protein Accession #: NP_001092.1
• Nucleotide Accession #: NM_001101.3