Catalog No: OKEH00008
Size:96T
Price: $425.00
SKU
OKEH00008
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ACE ELISA Kit (Rat) : 96 Wells (OKEH00008)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACE ELISA Kit (Rat) (OKEH00008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACE captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.0% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
ACE Microplate96 Wells (12 x 8 Well strips)
ACE Lyophilized Standard2
100X Biotinylated ACE Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.157 ng/mL
SpecificityNatural and recombinant Rat Angiotensin-converting enzyme
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAce
Gene Full Nameangiotensin I converting enzyme
Alias Symbolsangiotensin 1 converting enzyme 1;angiotensin I converting enzyme (peptidyl-dipeptidase A) 1;angiotensin I converting enzyme 1;angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1);angiotensin-converting enzyme;angiotensin-converting enzyme-like;CD143;Dcp1;Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme);dipeptidyl carboxypeptidase I;kininase II;StsRR92.
NCBI Gene Id24310
Protein NameP47820
Description of TargetAngiotensin-converting enzyme
Uniprot IDhttps://www.uniprot.org/uniprot/P47820
Protein Accession #NP_036676
Nucleotide Accession #NM_012544
Protein Size (# AA)1313
Molecular Weight151 kDa
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