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Catalog No: OKEH00336
Size:96T
Price: $600.00
SKU
OKEH00336
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ACAT1 ELISA Kit (Human) : 96 Wells (OKEH00336)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACAT1 ELISA Kit (Human) (OKEH00336) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACAT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for ACAT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACAT1 captured in well.
ELISA Kit Range7.8-500pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.1% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
ACAT1 Microplate96 Wells (12 x 8 Well strips)
ACAT1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ACAT1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity3.5 pg/mL
SpecificityNatural and recombinant Human Acetyl-CoA acetyltransferase, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACAT1
Gene Full Nameacetyl-CoA acetyltransferase 1
Alias SymbolsACAT;acetoacetyl Coenzyme A thiolase;acetoacetyl-CoA thiolase;acetyl-CoA acetyltransferase, mitochondrial;acetyl-Coenzyme A acetyltransferase 1;MAT;mitochondrial acetoacetyl-CoA thiolase;T2;testicular tissue protein Li 198;THIL.
NCBI Gene Id38
Protein NameP24752
Description of TargetAcetyl-CoA acetyltransferase, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P24752
Protein Accession #NP_000010.1
Nucleotide Accession #NM_000019.3
Protein Size (# AA)427
Molecular Weight45 kDa
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