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Catalog No: OKEH04621
Size:96T
Price: $675.00
SKU
OKEH04621
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology ACAN ELISA Kit (Dog) (OKEH04621) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACAN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ACAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACAN captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-ACAN Microplate96 Wells (12 x 8 Well Strips)
ACAN Lyophilized Standard2
Sample Diluent1 x 20 mL
ACAN Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.079 ng/mL
SpecificityNatural and recombinant Dog Aggrecan core protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACAN
Alias SymbolsCartilage-specific proteoglycan core protein.
NCBI Gene Id403828
Protein NameQ28343
Description of TargetAggrecan core protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q28343
Protein Accession #NP_001106926.2
Nucleotide Accession #NM_001113455.2
Protein Size (# AA)2333
Molecular Weight241 KDa
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