ACADM Antibody (OAEB02415)

Catalog No: OAEB02415 (Formerly GWB-4BE172)

Size:100UG

OAEB02415 staining (0.05ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02415 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ACADM
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 76%; Dog: 76%; Guinea Pig: 83%; Horse: 85%; Human: 100%; Mouse: 85%; Rabbit: 76%; Rat: 85%
• Peptide Sequence: C-RLIVAREHIDKYKN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32000.
  Western Blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1ug/ml.
  Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5ug/ml.
• Reference: * Powelka AM, Seth A, Virbasius JV, Kiskinis E, Nicoloro SM, Guilherme A, Tang X, Straubhaar J, Cherniack AD, Parker MG, Czech MP.; Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes.; J Clin Invest. 2006 Jan;116(1):125-36. Epub 2005 Dec 22.; Pubmed 16374519
  * Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K.; Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1.; Proc Natl Acad Sci U S A. 1986 Sep;83(17):6543-7.; Pubmed 3462713

Target Info

• Gene Symbol: ACADM
• Gene Full Name: acyl-CoA dehydrogenase medium chain
• Alias Symbols: ACAD1;acyl-CoA dehydrogenase, C-4 to C-12 straight chain;acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain;MCAD;MCADH;medium-chain acyl-CoA dehydrogenase;medium-chain specific acyl-CoA dehydrogenase, mitochondrial;testicular tissue protein Li 7.
• NCBI Gene Id: 34
• Protein Name: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
• Uniprot ID: P11310
• Protein Accession #: NP_000007.1
• Molecular Weight: 46.6; 47.0