ABP1 ELISA Kit (Mouse) (OKCD02487)

Catalog No: OKCD02487

Size:96WELLS

Mouse ABP1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-89%	85-94%	79-101%	90-104%
  EDTA Plasma (n=5)	78-91%	89-103%	78-92%	97-104%
  Heparin Plasma (n=5)	94-101%	98-105%	97-105%	83-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Binding Protein 1 (ABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Binding Protein 1 (ABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Binding Protein 1 (ABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Binding Protein 1 (ABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-102	94
  EDTA Plasma (n=5)	87-102	96
  Heparin Plasma(n=5)	92-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ABP1 Microplate: 96 Wells (12 x 8 Well strips)
  • ABP1 Lyophilized Standard: 2
  • 100X Biotinylated ABP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 13.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Diamine Oxidase (DAO). No significant cross-reactivity or interference between Diamine Oxidase (DAO) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABP1
• Gene Full Name: amine oxidase, copper-containing 1
• Alias Symbols: Amiloride-binding protein 1;Amine oxidase copper domain-containing protein 1;Histaminase.
• Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Description of Target: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.
• Uniprot ID: Q8JZQ5
• Protein Accession #: NP_001155093.1
• Nucleotide Accession #: NM_001161621.1
• Protein Size (# AA): 751