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Catalog No: OKEH02625
Size:96T
Price: $525.00
SKU
OKEH02625
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ABP1 ELISA Kit (Human) : 96 Wells (OKEH02625)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02625
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ABP1 ELISA Kit (Human) (OKEH02625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ABP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABP1 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 9.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ABP1 Microplate96 Wells (12 x 8 Well strips)
ABP1 Lyophilized Standard2
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
100X Biotinylated ABP1 Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.56 ng/mL
SpecificityNatural and recombinant Human Amiloride-sensitive amine oxidase [copper-containing]
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAOC1
Gene Full Nameamine oxidase copper containing 1
Alias SymbolsABP;ABP1;amiloride binding protein 1 (amine oxidase (copper-containing));amiloride-binding protein 1;amiloride-sensitive amine oxidase;amiloride-sensitive amine oxidase [copper-containing];amine oxidase copper domain-containing protein 1;DAO;DAO1;diamine oxidase;histaminase;KAO;kidney amine oxidase.
NCBI Gene Id26
Protein NameP19801
Description of TargetAmiloride-sensitive amine oxidase [copper-containing]
Uniprot IDhttps://www.uniprot.org/uniprot/P19801
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