ABI1 ELISA Kit (Human) (OKCD00577)

Catalog No: OKCD00577 (Formerly GWB-KBBIP5)

Size:96WELLS

Human ABI1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	96-105%	86-93%	84-99%	95-103%
  EDTA Plasma (n=5)	79-89%	78-94%	92-99%	94-101%
  Heparin Plasma (n=5)	78-104%	84-98%	81-96%	79-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Abl Interactor 1 (ABI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Abl Interactor 1 (ABI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Abl Interactor 1 (ABI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Abl Interactor 1 (ABI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-104	95
  EDTA plasma(n=5)	94-105	99
  heparin plasma(n=5)	87-101	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ABI1 Microplate: 96 Wells (12 x 8 Well Strips)
  • ABI1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ABI1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Abl Interactor 1 (ABI1). No significant cross-reactivity or interference between Abl Interactor 1 (ABI1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ABI1
• Gene Full Name: abl interactor 1
• Alias Symbols: Abelson interactor 1;ABI-1;abl interactor 1;abl-binding protein 4;ABLBP4;Abl-interactor protein 1 long;E3B1;eps8 SH3 domain-binding protein;interactor protein AblBP4;nap1 binding protein;Nap1-binding protein;NAP1BP;spectrin SH3 domain-binding protein 1;SSH3BP;SSH3BP1.
• NCBI Gene Id: 10006
• Protein Name: Abl interactor 1
• Description of Target: May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
• Uniprot ID: Q8IZP0
• Protein Accession #: NP_001012768
• Nucleotide Accession #: NM_001012750.2
• Protein Size (# AA): 508