Catalog No: OKEH03722
Size:96T
Price: $625.00
SKU
OKEH03722
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ABCG3 ELISA Kit (Mouse) (OKEH03722) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcg3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Abcg3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcg3 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
Abcg3 Microplate96 Wells (12 x 8 Well strips)
Abcg3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Abcg3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.179 ng/mL
SpecificityNatural and recombinant Mouse ATP-binding cassette sub-family G member 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAbcg3
Gene Full NameATP binding cassette subfamily G member 3
Alias SymbolsAbc;Abcp2;ATP-binding cassette sub-family G member 3;ATP-binding cassette, sub-family G (WHITE), member 3;Mxr;Mxr2.
NCBI Gene Id27405
Protein NameQ99P81
Description of TargetATP-binding cassette sub-family G member 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q99P81
Protein Accession #NP_084515.2
Nucleotide Accession #NM_030239.2
Protein Size (# AA)650
Molecular Weight74 kDa
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