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Catalog No: OKEH02082
Size:96T
Price: $525.00
SKU
OKEH02082
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ABCC6 ELISA Kit (Human) : 96 Wells (OKEH02082)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ABCC6 ELISA Kit (Human) (OKEH02082) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC6 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
ABCC6 Microplate96 Wells (12 x 8 Well strips)
ABCC6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PTPRQ-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity21 pg/mL
SpecificityNatural and recombinant Human Multidrug resistance-associated protein 6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolABCC6
Gene Full NameATP binding cassette subfamily C member 6
Alias SymbolsABC34;anthracycline resistance-associated protein;ARA;ATP-binding cassette sub-family C member 6;ATP-binding cassette, sub-family C (CFTR/MRP), member 6;EST349056;GACI2;MLP1;MOATE;MOAT-E;MRP6;multidrug resistance-associated protein 6;multi-specific organic anion transporter E;PXE;PXE1;URG7;URG7 protein.
NCBI Gene Id368
Protein NameO95255
Description of TargetMultidrug resistance-associated protein 6
Uniprot IDhttps://www.uniprot.org/uniprot/O95255
Protein Accession #NP_001072996.1
Nucleotide Accession #NM_001079528.3
Protein Size (# AA)1503
Molecular Weight165 kDa
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