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Catalog No: OKCD01208 (Formerly GWB-KBBHM6)
Size:96WELLS
Price: $700.00
SKU
OKCD01208
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Datasheets/ManualsPrintable datasheet for ABCA13 ELISA Kit (Mouse) (OKCD01208)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
90-101%83-104%81-98%91-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A13 (ABCA13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A13 (ABCA13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A13 (ABCA13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter A13 (ABCA13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A13 (ABCA13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A13 (ABCA13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Abca13 Microplate96 Wells (12 x 8 Well Strips)
Abca13 Lyophilized Standard2 x 5 ng
100X Biotinylated Abca13 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 33 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A13 (ABCA13). No significant cross-reactivity or interference between ATP Binding Cassette Transporter A13 (ABCA13) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAbca13
Gene Full NameATP-binding cassette, sub-family A (ABC1), member 13
Alias Symbols9830132L24;A930002G16Rik;AI956815;ATP-binding cassette sub-family A member 13;ATP-binding cassette transporter.
NCBI Gene Id268379
Protein NameATP-binding cassette sub-family A member 13
Description of TargetATP-binding cassette sub-family A member 13
Uniprot IDQ5SSE9
Protein Accession #NP_839990
Nucleotide Accession #NM_178259.3
Protein Size (# AA)5034
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