Catalog No: OKCA01963
Size:96 Wells
Price: $857.00
SKU
OKCA01963
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Sheep | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology AANAT ELISA Kit (Sheep) (OKCA01963) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AANAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AANAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AANAT captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 78.1 - 5,000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Lysates | ||||||||||||||||||||||
Sensitivity | 19.5 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | AANAT |
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Gene Full Name | Serotonin N-acetyltransferase |
Alias Symbols | AA-NAT |
NCBI Gene Id | 443531 |
Protein Name | Serotonin N-acetyltransferase |
Description of Target | Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin. |
Uniprot ID | Q29495 |
Protein Accession # | NP_001009461.1 |
Nucleotide Accession # | NM_001009461.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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