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Catalog No: OKCA01963
Size:96 Wells
Price: $857.00
SKU
OKCA01963
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivitySheep
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology AANAT ELISA Kit (Sheep) (OKCA01963) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AANAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AANAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AANAT captured in the well.
ELISA Kit Range78.1 - 5,000 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10091-105
EDTA Plasma (n=4)9385-97
ELISA Kit Component
ComponentAmount
AANAT Microplate96 Wells (12 x 8 Well strips)
AANAT Lyophilized Standard2 x 5 ng
100X Biotinylated AANAT Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity19.5 pg/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolAANAT
Gene Full NameSerotonin N-acetyltransferase
Alias SymbolsAA-NAT
NCBI Gene Id443531
Protein NameSerotonin N-acetyltransferase
Description of TargetControls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Uniprot IDQ29495
Protein Accession #NP_001009461.1
Nucleotide Accession #NM_001009461.1
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