A2ML1 ELISA Kit (Human) (OKCD01273)

Catalog No: OKCD01273 (Formerly GWB-KBBMN3)

Size:96WELLS

Human A2ML1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-97%	80-90%	78-103%	78-95%
  EDTA plasma(n=5)	83-101%	78-98%	78-101%	87-95%
  heparin plasma(n=5)	80-91%	84-101%	84-92%	96-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin Like Protein 1 (a2ML1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin Like Protein 1 (a2ML1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-2-Macroglobulin Like Protein 1 (a2ML1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-103	98
  EDTA plasma(n=5)	99-105	102
  heparin plasma(n=5)	79-91	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin Like Protein 1 (a2ML1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin Like Protein 1 (a2ML1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-A2ML1 Microplate: 96 Wells (12 x 8 Well Strips)
  • A2ML1 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated A2ML1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.34 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin Like Protein 1 (a2ML1). No significant cross-reactivity or interference between Alpha-2-Macroglobulin Like Protein 1 (a2ML1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: A2ML1
• Gene Full Name: alpha-2-macroglobulin like 1
• Alias Symbols: alpha-2-macroglobulin-like protein 1;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9;C3 and PZP-like, alpha-2-macroglobulin domain containing 9;CPAMD9;OMS;p170.
• NCBI Gene Id: 144568
• Protein Name: Alpha-2-macroglobulin-like protein 1
• Description of Target: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.
• Uniprot ID: A8K2U0
• Protein Accession #: NP_001269353
• Nucleotide Accession #: NM_001282424.2
• Protein Size (# AA): 1454