A2M ELISA Kit (Bovine) (OKEH03891)

Catalog No: OKEH03891

Size:96T

Bovine A2M ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology A2M ELISA Kit (Bovine) (OKEH03891) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A2M has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for A2M is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A2M captured in well.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Component:
  • Anti-A2M Microplate: 96 Wells (12 x 8 Well strips)
  • A2M Lyophilized Standard: 2
  • 100X Biotinylated A2M Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 1.47 ng/mL
• Specificity: Natural and recombinant Bovine Alpha-2-macroglobulin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: A2M
• Gene Full Name: alpha-2-macroglobulin
• Alias Symbols: alpha-2-M;alpha-2-macroglobulin;alpha-2-macroglobulin variant 10;alpha-2-macroglobulin variant 11;alpha-2-macroglobulin variant 12;alpha-2-macroglobulin variant 13;alpha-2-macroglobulin variant 14;alpha-2-macroglobulin variant 15;alpha-2-macroglobulin variant 16;alpha-2-macroglobulin variant 17;alpha-2-macroglobulin variant 18;alpha-2-macroglobulin variant 19;alpha-2-macroglobulin variant 2;alpha-2-macroglobulin variant 21;alpha-2-macroglobulin variant 23;alpha-2-macroglobulin variant 3;alpha-2-macroglobulin variant 4;alpha-2-macroglobulin variant 5;alpha-2-macroglobulin variant 6;alpha-2-macroglobulin variant 7;alpha-2-macroglobulin variant 8;alpha-2-macroglobulin variant 9.
• NCBI Gene Id: 513856
• Protein Name: Q7SIH1
• Description of Target: Alpha-2-macroglobulin
• Uniprot ID: https://www.uniprot.org/uniprot/Q7SIH1
• Protein Accession #: NP_001103265.1
• Nucleotide Accession #: NM_001109795.1
• Protein Size (# AA): 1510
• Molecular Weight: 168 kDa