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Catalog No: OKCD02082 (Formerly GWB-KBBMV9)
Size:96WELLS
Price: $750.00
SKU
OKCD02082
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Datasheets/ManualsPrintable datasheet for Wnt16 ELISA Kit (Mouse) (OKCD02082)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)94-103%89-103%98-105%96-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 16 (WNT16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 16 (WNT16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 16 (WNT16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 16 (WNT16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum81-10193
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 16 (WNT16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 16 (WNT16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Wnt16 Microplate96 Wells (12 x 8 Well Strips)
Wnt16 Lyophilized Standard2 x 5 ng
100X Biotinylated Wnt16 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 28 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 16 (WNT16). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 16 (WNT16) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolWnt16
Gene Full Namewingless-type MMTV integration site family, member 16
Alias SymbolsE130309I19Rik;protein Wnt-16;wingless-related MMTV integration site 16.
NCBI Gene Id93735
Protein NameProtein Wnt-16
Description of TargetLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Uniprot IDQ9QYS1
Protein Accession #NP_444346.3
Nucleotide Accession #NM_053116.4
Protein Size (# AA)364
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