WDR35 ELISA Kit (Human) (OKCD02112)

Catalog No: OKCD02112 (Formerly GWB-KBBTU8)

Size:96WELLS

Human WDR35 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WD Repeat Containing Domain Protein 35 (WDR35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WD Repeat Containing Domain Protein 35 (WDR35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WD Repeat Containing Domain Protein 35 (WDR35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WD Repeat Containing Domain Protein 35 (WDR35) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WD Repeat Containing Domain Protein 35 (WDR35) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WD Repeat Containing Domain Protein 35 (WDR35) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-WDR35 Microplate: 96 Wells (12 x 8 Well Strips)
  • WDR35 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated WDR35 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of WD Repeat Containing Domain Protein 35 (WDR35). No significant cross-reactivity or interference between WD Repeat Containing Domain Protein 35 (WDR35) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: WDR35
• Gene Full Name: WD repeat domain 35
• Alias Symbols: CED2, IFTA1, SRTD7, FAP118, IFT121
• NCBI Gene Id: 57539
• Protein Name: WD repeat-containing protein 35
• Description of Target: Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport. Required for ciliogenesis. May promote CASP3 activation and TNF-stimulated apoptosis.
• Uniprot ID: Q9P2L0
• Protein Accession #: NP_001006658.1
• Nucleotide Accession #: NM_001006657.1
• Protein Size (# AA): 1181