UGT2B7 ELISA Kit (Human) (OKCD01937)

Catalog No: OKCD01937 (Formerly GWB-KBBIM7)

Size:96WELLS

Human UGT2B7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	83-102%	96-105%	95-102%	98-105%
  EDTA Plasma (n=5)	85-104%	95-102%	89-101%	99-105%
  Heparin Plasma (n=5)	82-98%	91-99%	96-104%	82-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-95	87
  EDTA Plasma (n=5)	80-102	86
  Heparin Plasma (n=5)	90-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-UGT2B7 Microplate: 96 Wells (12 x 8 Well Strips)
  • UGT2B7 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated UGT2B7 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). No significant cross-reactivity or interference between UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: UGT2B7
• Gene Full Name: UDP glucuronosyltransferase family 2 member B7
• Alias Symbols: 3,4-catechol estrogen specific;3,4-catechol estrogen-specific UDPGT;UDP glucuronosyltransferase 2 family, polypeptide B7;UDP-glucuronosyltransferase 2B7;UDP-glucuronosyltransferase 2B9;UDP-glucuronyltransferase, family 2, beta-7;UDPGT 2B7;UDPGT 2B9;UDPGT2B7;UDPGTh-2;UDPGTH2;UGT2B9.
• NCBI Gene Id: 7364
• Protein Name: P16662
• Description of Target: UDP-glucuronosyltransferase 2B7
• Uniprot ID: https://www.uniprot.org/uniprot/P16662
• Protein Accession #: NP_001065.2
• Nucleotide Accession #: NM_001074.2
• Protein Size (# AA): 529