UBIAD1 ELISA Kit (Human) (OKCD02060)

Catalog No: OKCD02060 (Formerly GWB-KBBKZ5)

Size:96WELLS

Human UBIAD1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-103%	98-105%	87-101%	86-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	84-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-UBIAD1 Microplate: 96 Wells (12 x 8 Well Strips)
  • UBIAD1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated UBIAD1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.067 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1). No significant cross-reactivity or interference between UbiA Prenyltransferase Domain Containing Protein 1 (UBIAD1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: UBIAD1
• Gene Full Name: UbiA prenyltransferase domain containing 1
• Alias Symbols: SCCD, TERE1
• NCBI Gene Id: 29914
• Protein Name: UbiA prenyltransferase domain-containing protein 1
• Description of Target: Prenyltransferase that mediates the formation of menaquinone-4 (MK-4) and coenzyme Q10. MK-4 is a vitamin K2 isoform present at high concentrations in the brain, kidney and pancreas, and is required for endothelial cell development. Mediates the conversion of phylloquinone (PK) into MK-4, probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. Also plays a role in cardiovascular development independently of MK-4 biosynthesis, by acting as a coenzyme Q10 biosyntetic enzyme: coenzyme Q10, also named ubiquinone, plays a important antioxidant role in the cardiovascular system. Mediates biosynthesis of coenzyme Q10 in the Golgi membrane, leading to protect cardiovascular tissues from NOS3/eNOS-dependent oxidative stress.
• Uniprot ID: Q9Y5Z9
• Protein Accession #: NP_037451.1
• Nucleotide Accession #: NM_013319.2
• Protein Size (# AA): 338