UBE2C ELISA Kit (Human) (OKCD01828)

Catalog No: OKCD01828 (Formerly GWB-KBBXZ5)

Size:96WELLS

Human UBE2C ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2C (UBE2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2C (UBE2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2C (UBE2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ubiquitin Conjugating Enzyme E2C (UBE2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2C (UBE2C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2C (UBE2C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-UBE2C Microplate: 96 Wells (12 x 8 Well Strips)
  • UBE2C Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated UBE2C Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2C (UBE2C). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2C (UBE2C) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: UBE2C
• Gene Full Name: ubiquitin conjugating enzyme E2 C
• Alias Symbols: (E3-independent) E2 ubiquitin-conjugating enzyme C;cyclin-selective ubiquitin carrier protein;dJ447F3.2;E2 ubiquitin-conjugating enzyme C;mitotic-specific ubiquitin-conjugating enzyme;UBCH10;Ubiquitin carrier protein C;ubiquitin conjugating enzyme E2C;ubiquitin-conjugating enzyme E2 C;ubiquitin-protein ligase C.
• NCBI Gene Id: 11065
• Protein Name: Ubiquitin-conjugating enzyme E2 C
• Description of Target: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
• Uniprot ID: O00762
• Protein Accession #: NP_001268670.1
• Nucleotide Accession #: NM_001281741.1
• Protein Size (# AA): 179