TSG101 ELISA Kit (Human) (OKCD01703)

Catalog No: OKCD01703 (Formerly GWB-KBBFW6)

Size:96WELLS

Human TSG101 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	80-93%	96-103%	84-92%	91-98%
  EDTA Plasma (n=5)	95-102%	86-101%	91-99%	79-98%
  Heparin Plasma (n=5)	98-105%	80-93%	82-102%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Susceptibility Gene 101 (TSG101). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Susceptibility Gene 101 (TSG101). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Susceptibility Gene 101 (TSG101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Susceptibility Gene 101 (TSG101) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	78-98	91
  EDTA Plasma (n=5)	86-101	93
  Heparin Plasma (n=5)	78-88	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TSG101 Microplate: 96 Wells (12 x 8 Well Strips)
  • TSG101 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TSG101 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Susceptibility Gene 101 (TSG101). No significant cross-reactivity or interference between Tumor Susceptibility Gene 101 (TSG101) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TSG101
• Gene Full Name: tumor susceptibility 101
• Alias Symbols: ESCRT-I complex subunit TSG101;TSG10;tumor susceptibility gene 10;tumor susceptibility gene 101;tumor susceptibility gene 101 protein;tumor susceptibility protein;VPS23.
• NCBI Gene Id: 7251
• Protein Name: Tumor susceptibility gene 101 protein
• Description of Target: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).
• Uniprot ID: Q99816
• Protein Accession #: NP_006283.1
• Nucleotide Accession #: NM_006292.3
• Protein Size (# AA): 390