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Catalog No: OKCD01615 (Formerly GWB-KBBIC1)
Size:96WELLS
Price: $650.00
SKU
OKCD01615
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Datasheets/ManualsPrintable datasheet for TNXB ELISA Kit (Human) (OKCD01615)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-96%88-101%89-102%85-93%
EDTA Plasma (n=5)81-98%90-105%87-96%92-105%
Heparin Plasma (n=5)78-103%97-105%89-97%88-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tenascin X (TNX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tenascin X (TNX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tenascin X (TNX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tenascin X (TNX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-9793
EDTA Plasma (n=5)89-10393
Heparin Plasma (n=5)96-104101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tenascin X (TNX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tenascin X (TNX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TNXB Microplate96 Wells (12 x 8 Well Strips)
TNXB Lyophilized Standard2 x 10 ng
100X Biotinylated TNXB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tenascin X (TNX). No significant cross-reactivity or interference between Tenascin X (TNX) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTNXB
Gene Full Nametenascin XB
Alias SymbolsEDS3;EDSCLL;EDSCLL1;growth-inhibiting protein 45;hexabrachion-like protein;HXBL;tenascin XB1;tenascin XB2;tenascin-X;TENX;TNX;TN-X;TNXB1;TNXB2;TNXBS;VUR8;XB;XBS.
NCBI Gene Id7148
Protein NameTenascin-X
Description of TargetAppears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors.
Uniprot IDP22105
Protein Accession #NP_061978.6
Nucleotide Accession #NM_019105.6
Protein Size (# AA)4242
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