TMPRSS6 ELISA Kit (Human) (OKCD01883)

Catalog No: OKCD01883 (Formerly GWB-KBBHD2)

Size:96WELLS

Human TMPRSS6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protease, Serine 6 (TMPRSS6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protease, Serine 6 (TMPRSS6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protease, Serine 6 (TMPRSS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protease, Serine 6 (TMPRSS6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transmembrane Protease, Serine 6 (TMPRSS6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transmembrane Protease, Serine 6 (TMPRSS6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TMPRSS6 Microplate: 96 Wells (12 x 8 Well Strips)
  • TMPRSS6 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated TMPRSS6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transmembrane Protease, Serine 6 (TMPRSS6). No significant cross-reactivity or interference between Transmembrane Protease, Serine 6 (TMPRSS6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TMPRSS6
• Gene Full Name: transmembrane serine protease 6
• Alias Symbols: IRIDA;matriptase-2;membrane-bound mosaic serine proteinase matriptase-2;MT2;transmembrane protease serine 6;transmembrane protease, serine 6;type II transmembrane serine protease 6.
• NCBI Gene Id: 164656
• Protein Name: Transmembrane protease serine 6
• Description of Target: Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Through the cleavage of HFE2, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis.
• Uniprot ID: Q8IU80
• Protein Accession #: NP_001275929.1
• Nucleotide Accession #: NM_001289000.1
• Protein Size (# AA): 811