TMEM176B ELISA Kit (Human) (OKCD02076)

Catalog No: OKCD02076 (Formerly GWB-KBBJB3)

Size:96WELLS

Human TMEM176B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 176B (TMEM176B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 176B (TMEM176B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 176B (TMEM176B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protein 176B (TMEM176B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transmembrane Protein 176B (TMEM176B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transmembrane Protein 176B (TMEM176B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TMEM176B Microplate: 96 Wells (12 x 8 Well Strips)
  • TMEM176B Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated TMEM176B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 176B (TMEM176B). No significant cross-reactivity or interference between Transmembrane Protein 176B (TMEM176B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TMEM176B
• Gene Full Name: Transmembrane protein 176B
• Alias Symbols: LR8, MS4B2
• NCBI Gene Id: 28959
• Protein Name: Transmembrane protein 176B
• Description of Target: May play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells.
• Uniprot ID: Q3YBM2
• Protein Accession #: NP_001094781.1
• Nucleotide Accession #: NM_001101311.1
• Protein Size (# AA): 270