TIMP2 ELISA Kit (Horse) (OKCD01484)

Catalog No: OKCD01484 (Formerly GWB-KBBMZ4)

Size:96WELLS

Horse TIMP2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Equus caballus|Horse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	81-90%	88-95%	98-105%	88-98%
  EDTA Plasma (n=5)	86-101%	84-92%	89-102%	87-104%
  Heparin Plasma (n=5)	97-104%	87-101%	80-95%	79-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-93	89
  EDTA Plasma (n=5)	94-103	98
  Heparin Plasma (n=5)	84-104	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TIMP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • TIMP2 Lyophilized Standard: 2 x 500 ng
  • 100X Biotinylated TIMP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 2.9 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TIMP2
• Alias Symbols: Tissue inhibitor of metalloproteinases 2.
• NCBI Gene Id: 100034134
• Protein Name: Metalloproteinase inhibitor 2
• Description of Target: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them.
• Uniprot ID: O77717
• Protein Accession #: XP_014593727.1
• Nucleotide Accession #: XM_014738241.1
• Protein Size (# AA): 91