TGM6 ELISA Kit (Human) (OKCD01939)

Catalog No: OKCD01939 (Formerly GWB-KBBTO3)

Size:96WELLS

Human TGM6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-95%	78-104%	78-94%	98-105%
  EDTA Plasma (n=5)	97-104%	90-97%	87-98%	88-102%
  Heparin Plasma (n=5)	81-92%	87-96%	98-105%	85-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 6 (TGM6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 6 (TGM6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 6 (TGM6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 6 (TGM6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	81-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 6 (TGM6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 6 (TGM6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TGM6 Microplate: 96 Wells (12 x 8 Well Strips)
  • TGM6 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TGM6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.060 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transglutaminase 6 (TGM6). No significant cross-reactivity or interference between Transglutaminase 6 (TGM6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TGM6
• Gene Full Name: transglutaminase 6
• Alias Symbols: dJ734P14.3;protein-glutamine gamma-glutamyltransferase 6;SCA35;TG6;TGase Y;TGase-6;TGM3L;TGY;transglutaminase y;Transglutaminase-3-like;Transglutaminase-6.
• NCBI Gene Id: 343641
• Protein Name: Protein-glutamine gamma-glutamyltransferase 6
• Description of Target: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
• Uniprot ID: O95932
• Protein Accession #: NP_001241663.1
• Nucleotide Accession #: NM_001254734.1
• Protein Size (# AA): 706