TGM1 ELISA Kit (Human) (OKCD01601)

Catalog No: OKCD01601 (Formerly GWB-KBBRE5)

Size:96WELLS

Human TGM1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 1 (TGM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 1 (TGM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 1 (TGM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transglutaminase 1 (TGM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 1 (TGM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 1 (TGM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TGM1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TGM1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TGM1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transglutaminase 1, Keratinocyte (TGM1). No significant cross-reactivity or interference between Transglutaminase 1, Keratinocyte (TGM1) and analogues was observed.
• Publications:

  Polarization of protease-activated receptor 2 (PAR-2) signaling is altered during airway epithelial remodeling and deciliation. J Biol Chem. 295, 6721-6740 (2020). 32241907

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for TGM1 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: TGM1
• Gene Full Name: transglutaminase 1
• Alias Symbols: ARCI1;epidermal TGase;ICR2;K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase;KTG;LI;LI1;protein-glutamine gamma-glutamyltransferase K;TG(K);TGASE;TGase K;TGase-1;TGK;transglutaminase K;transglutaminase, keratinocyte;Transglutaminase-1.
• NCBI Gene Id: 7051
• Protein Name: Protein-glutamine gamma-glutamyltransferase K
• Description of Target: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation (PubMed:26220141).
• Uniprot ID: P22735
• Protein Accession #: NP_000350.1
• Nucleotide Accession #: NM_000359.2
• Protein Size (# AA): 817