TERF2IP ELISA Kit (Human) (OKCD02027)

Catalog No: OKCD02027 (Formerly GWB-KBBLQ9)

Size:96WELLS

Human TERF2IP ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TERF2IP Microplate: 96 Wells (12 x 8 Well Strips)
  • TERF2IP Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated TERF2IP Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.66 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP). No significant cross-reactivity or interference between Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TERF2IP
• Gene Full Name: TERF2 interacting protein
• Alias Symbols: RAP1, DRIP5
• NCBI Gene Id: 54386
• Protein Name: Telomeric repeat-binding factor 2-interacting protein 1
• Description of Target: Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
• Uniprot ID: Q9NYB0
• Protein Accession #: NP_061848.2
• Nucleotide Accession #: NM_018975.3
• Protein Size (# AA): 399