TCIRG1 ELISA Kit (Human) (OKCD02071)

Catalog No: OKCD02071 (Formerly GWB-KBBYZ7)

Size:96WELLS

Human TCIRG1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	97-105%	79-92%	98-105%	89-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1 (TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of T-Cell, Immune Regulator 1 (TCIRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	79-89	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TCIRG1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TCIRG1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated TCIRG1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum and other biological fluids
• Sensitivity: < 6.0 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of T-Cell, Immune Regulator 1 (TCIRG1). No significant cross-reactivity or interference between T-Cell, Immune Regulator 1 (TCIRG1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TCIRG1
• Gene Full Name: T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
• Alias Symbols: a3;Atp6i;ATP6N1C;ATP6V0A3;ATPase, H+ transporting, 116kD;OC116;OC-116kDa;OPTB1;osteoclastic proton pump 116 kDa subunit;specific 116-kDa vacuolar proton pump subunit;Stv1;T-cell immune regulator 1;T-cell immune response cDNA 7;T-cell immune response cDNA7 protein;T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a;T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3;TIRC7;vacuolar proton translocating ATPase 116 kDa subunit A;Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3;V-ATPase 116 kDa subunit a3;V-ATPase 116-kDa;V-ATPase subunit a3;Vph1;V-type proton ATPase 116 kDa subunit a;V-type proton ATPase 116 kDa subunit a3.
• NCBI Gene Id: 10312
• Protein Name: V-type proton ATPase 116 kDa subunit a isoform 3
• Description of Target: Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation.
• Uniprot ID: Q13488
• Protein Accession #: NP_006010.2
• Nucleotide Accession #: NM_006019.3
• Protein Size (# AA): 830