Spink5 ELISA Kit (Mouse) (OKCD01946)

Catalog No: OKCD01946 (Formerly GWB-KBBJN3)

Size:96WELLS

Mouse Spink5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Spink5 Microplate: 96 Wells (12 x 8 Well Strips)
  • Spink5 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Spink5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 29 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). No significant cross-reactivity or interference between Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Spink5
• Gene Full Name: serine peptidase inhibitor, Kazal type 5
• Alias Symbols: 2310065D10Rik;AV238912;LEKT;LEKT1;LETK1;Netherton syndrome;serine protease inhibitor Kazal-type 5;serine protease inhibitor, Kazal type 5;VAKT1.
• NCBI Gene Id: 72432
• Protein Name: Serine peptidase inhibitor, Kazal type 5
• Description of Target: Serine peptidase inhibitor, Kazal type 5
• Uniprot ID: Q148R4
• Protein Accession #: NP_001074649.1
• Nucleotide Accession #: NM_001081180.1
• Protein Size (# AA): 1017