Catalog No: OKCD01706 (Formerly GWB-KBBKP7)
Size:96WELLS
Price: $725.00
SKU
OKCD01706
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Datasheets/ManualsPrintable datasheet for Sfrp5 ELISA Kit (Rat) (OKCD01706)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-105%83-90%97-105%87-96%
EDTA Plasma (n=5)81-93%87-98%89-103%91-103%
Heparin Plasma (n=5)98-105%78-90%80-102%88-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 5 (SFRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 5 (SFRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secreted Frizzled Related Protein 5 (SFRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)94-10198
EDTA Plasma (n=5)78-9684
Heparin Plasma (n=5)79-9895
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 5 (SFRP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Sfrp5 Microplate96 Wells (12 x 8 Well Strips)
Sfrp5 Lyophilized Standard2 x 8 ng
100X Biotinylated Sfrp5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 47 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 5 (SFRP5). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 5 (SFRP5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSfrp5
Gene Full Namesecreted frizzled-related protein 5
Alias Symbolssecreted frizzled-related protein 5;secreted frizzled-related sequence protein 5.
NCBI Gene Id309377
Protein NameSecreted frizzled-related protein 5
Description of TargetSecreted frizzled-related protein 5
Uniprot IDD3ZTV6
Protein Accession #NP_001101061.1
Nucleotide Accession #NM_001107591.1
Protein Size (# AA)314
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