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Catalog No: OKCD01897 (Formerly GWB-KBBEF9)
Size:96WELLS
Price: $675.00
SKU
OKCD01897
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Datasheets/ManualsPrintable datasheet for SYNRg ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synergin Gamma (SYNRg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synergin Gamma (SYNRg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synergin Gamma (SYNRg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Synergin Gamma (SYNRg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synergin Gamma (SYNRg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synergin Gamma (SYNRg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SYNRG Microplate96 Wells (12 x 8 Well Strips)
SYNRG Lyophilized Standard2 x 20 ng
100X Biotinylated SYNRG Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.109 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Synergin Gamma (SYNRg). No significant cross-reactivity or interference between Synergin Gamma (SYNRg) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSYNRG
Gene Full NameSynergin gamma
Alias SymbolsSYNG, AP1GBP1
NCBI Gene Id11276
Protein NameSynergin gamma
Description of TargetMay play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins.
Uniprot IDQ9UMZ2
Protein Accession #NP_001157016.1
Nucleotide Accession #NM_001163544.2
Protein Size (# AA)1314
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