STK39 ELISA Kit (Human) (OKCD02081)

Catalog No: OKCD02081 (Formerly GWB-KBBQW3)

Size:96WELLS

Human STK39 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 39 (STK39). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 39 (STK39). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 39 (STK39), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serine/Threonine Kinase 39 (STK39) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-STK39 Microplate: 96 Wells (12 x 8 Well Strips)
  • STK39 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated STK39 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.24 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 39 (STK39). No significant cross-reactivity or interference between Serine/Threonine Kinase 39 (STK39) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: STK39
• Gene Full Name: serine/threonine kinase 39
• Alias Symbols: DCHT;PASK;proline-alanine-rich STE20-related kinase;serine threonine kinase 39 (STE20/SPS1 homolog, yeast);serine/threonine-protein kinase 39;small intestine SPAK-like kinase;SPAK;STE20/SPS1 homolog;STE20/SPS1-related proline-alanine-rich protein kinase;Ste20-like protein kinase;ste-20-related kinase.
• NCBI Gene Id: 27347
• Protein Name: STE20/SPS1-related proline-alanine-rich protein kinase
• Description of Target: May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.
• Uniprot ID: Q9UEW8
• Protein Accession #: NP_037365.2
• Nucleotide Accession #: NM_013233.2
• Protein Size (# AA): 545