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Catalog No: OKCD02002 (Formerly GWB-KBBJF5)
Size:96WELLS
Price: $675.00
SKU
OKCD02002
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Datasheets/ManualsPrintable datasheet for SSH2 ELISA Kit (Human) (OKCD02002)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slingshot Homolog 2 (SSH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slingshot Homolog 2 (SSH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slingshot Homolog 2 (SSH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slingshot Homolog 2 (SSH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slingshot Homolog 2 (SSH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slingshot Homolog 2 (SSH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CILP2 Microplate96 Wells (12 x 8 Well Strips)
CILP2 Lyophilized Standard2 x 20 ng
100X Biotinylated CILP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 7 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Slingshot Homolog 2 (SSH2). No significant cross-reactivity or interference between Slingshot Homolog 2 (SSH2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSSH2
Gene Full Nameslingshot protein phosphatase 2
Alias Symbolsprotein phosphatase Slingshot homolog 2;SSH-2;SSH-2L;SSH-like protein 2.
NCBI Gene Id85464
Protein NameProtein phosphatase Slingshot homolog 2
Description of TargetProtein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Uniprot IDQ76I76
Protein Accession #NP_001269058.1
Nucleotide Accession #NM_001282129.1
Protein Size (# AA)1423
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