SPAM1 ELISA Kit (Human) (OKCA00696)

Catalog No: OKCA00696

Size:96 Wells

Human SPAM1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	94	90-98
  1:2	97	91-102
  1:4	90	85-94
  1:8	85	80-89

• ELISA Kit Principle: Aviva Systems Biology SPAM1 ELISA Kit (Human) (OKCA00696) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPAM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SPAM1 captured in the well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	104	100-108
  EDTA Plasma (n=4)	87	82-93

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level SPAM1 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level SPAM1 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • SPAM1 Microplate: 96 Wells (12 x 8 Well strips)
  • SPAM1 Lyophilized Standard: 2
  • 100X Biotinylated SPAM1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SPAM1
• Gene Full Name: Sperm adhesion molecule 1
• Alias Symbols: HYA1, PH20, HYAL1, HYAL3, HYAL5, PH-20, SPAG15, HEL-S-96n
• NCBI Gene Id: 6677
• Protein Name: Hyaluronidase PH-20
• Description of Target: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
• Uniprot ID: P38567
• Protein Accession #: NP_001167515.1
• Nucleotide Accession #: NM_001174044.1