Catalog No: OKCD01924 (Formerly GWB-KBBZF8)
Size:96WELLS
Price: $675.00
SKU
OKCD01924
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Datasheets/ManualsPrintable datasheet for SLC16A8 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-105%91-105%95-104%85-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 16, Member 8 (SLC16A8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 16, Member 8 (SLC16A8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 16, Member 8 (SLC16A8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Solute Carrier Family 16, Member 8 (SLC16A8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum88-9693
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 16, Member 8 (SLC16A8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 16, Member 8 (SLC16A8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SLC16A8 Microplate96 Wells (12 x 8 Well Strips)
SLC16A8 Lyophilized Standard2 x 20 ng
100X Biotinylated SLC16A8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.123 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 16, Member 8 (SLC16A8). No significant cross-reactivity or interference between Solute Carrier Family 16, Member 8 (SLC16A8) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSLC16A8
Gene Full NameSolute carrier family 16 member 8
Alias SymbolsMCT3, REMP
NCBI Gene Id23539
Protein NameMonocarboxylate transporter 3
Description of TargetProton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Uniprot IDO95907
Protein Accession #NP_037488.2
Nucleotide Accession #NM_013356.2
Protein Size (# AA)504
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