Catalog No: OKCA00806
Size:96 Wells
Price: $754.00
SKU
OKCA00806
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9591-98
1:28881-92
1:410498-111
1:89783-93
ELISA Kit PrincipleAviva Systems Biology SCGB3A2 ELISA Kit (Human) (OKCA00806) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCGB3A2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCGB3A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SCGB3A2 captured in the well.
ELISA Kit Range46.88 pg/mL-3,000 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9185-99
EDTA Plasma (n=4)9589-100
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level SCGB3A2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SCGB3A2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
SCGB3A2 Microplate96 Wells (12 x 8 Well strips)
SCGB3A2 Lyophilized Standard2
100X Biotinylated SCGB3A2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity11.72 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSCGB3A2
Gene Full NameSecretoglobin family 3A member 2
Alias SymbolsLU103, PNSP1, UGRP1, pnSP-1
NCBI Gene Id117156
Protein NameSecretoglobin family 3A member 2
Description of TargetThe protein encoded by this gene is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma.
Uniprot IDQ96PL1
Protein Accession #NP_473364.1
Nucleotide Accession #NM_054023.4
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