Catalog No: OKCD01525 (Formerly GWB-KBBVZ1)
Size:96WELLS
Price: $650.00
SKU
OKCD01525
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Datasheets/ManualsPrintable datasheet for S100B ELISA Kit (Rabbit) (OKCD01525)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-97%92-99%87-96%96-105%
EDTA Plasma (n=5)93-105%94-103%78-104%86-97%
Heparin Plasma (n=5)88-104%96-103%78-99%79-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein B (S100B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein B (S100B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein B (S100B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)86-9894
Heparin Plasma (n=5)89-9892
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein B (S100B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein B (S100B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-S100B Microplate96 Wells (12 x 8 Well Strips)
S100B Lyophilized Standard2 x 1 ng
100X Biotinylated S100B Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein B (S100B). No significant cross-reactivity or interference between S100 Calcium Binding Protein B (S100B) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolS100B
Gene Full NameS100 calcium binding protein B
Alias Symbolsprotein S100-B;S100 calcium-binding protein B;S-100 calcium-binding protein beta subunit;S-100 protein beta chain;S-100 protein subunit beta.
NCBI Gene Id100009495
Protein NameProtein S100-B
Description of TargetWeakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).
Uniprot IDQ6YNR6
Protein Accession #NP_001076199.1
Nucleotide Accession #NM_001082730.1
Protein Size (# AA)92
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