Rspo2 ELISA Kit (Mouse) (OKCD02065)

Catalog No: OKCD02065 (Formerly GWB-KBBDM2)

Size:96WELLS

Mouse Rspo2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	84-95%	82-97%	87-103%	81-97%
  EDTA Plasma (n=5)	78-99%	94-105%	91-101%	81-88%
  Heparin Plasma (n=5)	88-96%	79-91%	96-105%	92-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2 (RSPO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 2 (RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2 (RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of R-Spondin 2 (RSPO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	88-95	91
  EDTA Plasma (n=5)	88-95	91
  Heparin Plasma (n=5)	95-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Rspo2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Rspo2 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Rspo2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 29 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of R-Spondin 2 (RSPO2). No significant cross-reactivity or interference between R-Spondin 2 (RSPO2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Rspo2
• Gene Full Name: R-spondin 2
• Alias Symbols: 2610028F08Rik;AA673245;cristin-2;cysteine-rich and single thrombospondin domain-containing protein 2;D430027K22;ft;ftls;mCristin-2;roof plate-specific spondin-2;R-spondin 2 homolog;R-spondin-2.
• NCBI Gene Id: 239405
• Protein Name: R-spondin-2
• Description of Target: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.
• Uniprot ID: Q8BFU0
• Protein Accession #: NP_766403.1
• Nucleotide Accession #: NM_172815.3
• Protein Size (# AA): 243