RAB35 ELISA Kit (Human) (OKCA00795)

Catalog No: OKCA00795

Size:96 Wells

Human RAB35 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	92	89-96
  1:2	95	91-99
  1:4	97	92-102
  1:8	90	85-96

• ELISA Kit Principle: Aviva Systems Biology RAB35 ELISA Kit (Human) (OKCA00795) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB35 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB35 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RAB35 captured in the well.
• ELISA Kit Range: 47 pg/mL-3,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96	89-102
  EDTA Plasma (n=4)	94	90-98

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level RAB35 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level RAB35 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • RAB35 Microplate: 96 Wells (12 x 8 Well strips)
  • RAB35 Lyophilized Standard: 2
  • 100X Biotinylated RAB35 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 11.7 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RAB35
• Gene Full Name: RAB35, member RAS oncogene family
• Alias Symbols: RAY, H-ray, RAB1C
• NCBI Gene Id: 11021
• Protein Name: Ras-related protein Rab-35
• Description of Target: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.
• Uniprot ID: Q15286
• Protein Accession #: NP_001161078.1
• Nucleotide Accession #: NM_001167606.1