Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKCD01953 (Formerly GWB-KBBPX4)
Size:96WELLS
Price: $725.00
SKU
OKCD01953
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for PPM1A ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Ppm1a Microplate96 Wells (12 x 8 Well Strips)
Ppm1a Lyophilized Standard2 x 20 ng
100X Biotinylated Ppm1a Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.119 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). No significant cross-reactivity or interference between Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPPM1A
Gene Full NameProtein phosphatase, Mg2+/Mn2+ dependent, 1A
Alias SymbolsPP2CA, PP2Calpha, PP2C-ALPHA
NCBI Gene Id5494
Protein NameProtein phosphatase 1A
Description of TargetEnzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
Uniprot IDP20650
Protein Accession #NP_058734.1
Nucleotide Accession #NM_017038.1
Protein Size (# AA)382
Write Your Own Review
You're reviewing:Ppm1a ELISA Kit (Rat) (OKCD01953)
Your Rating
We found other products you might like!