Catalog No: OKCD01511 (Formerly GWB-KBBCH9)
Size:96WELLS
Price: $650.00
SKU
OKCD01511
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Datasheets/ManualsPrintable datasheet for PHB ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-93%98-105%86-101%92-99%
EDTA Plasma (n=5)85-102%97-105%82-93%87-96%
Heparin Plasma (n=5)92-101%84-98%81-101%87-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prohibitin (PHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prohibitin (PHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prohibitin (PHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prohibitin (PHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)96-104101
EDTA Plasma (n=5)91-10499
Heparin Plasma (n=5)80-9083
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prohibitin (PHB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prohibitin (PHB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Phb Microplate96 Wells (12 x 8 Well Strips)
Phb Lyophilized Standard2 x 20 ng
100X Biotinylated Phb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.121 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Prohibitin (PHB). No significant cross-reactivity or interference between Prohibitin (PHB) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPHB
Gene Full NameProhibitin
Alias SymbolsProhibitin
NCBI Gene Id25344
Protein NameProhibitin
Description of TargetProhibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Uniprot IDP67779
Protein Accession #NP_114039.1
Nucleotide Accession #NM_031851.2.
Protein Size (# AA)272
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